间皮素
胰腺癌
胰腺
腺癌
免疫组织化学
CA19-9号
病理
胰腺疾病
生物
基因表达的系列分析
癌症研究
癌症
医学
基因表达
基因表达谱
基因
间皮瘤
内分泌学
生物化学
遗传学
作者
Pedram Argani,Christine A. Iacobuzio‐Donahue,Byungwoo Ryu,Christophe Rosty,Michael Goggins,Robb E. Wilentz,Selva R. Murugesan,Steven D. Leach,Elizabeth M. Jaffee,Charles J. Yeo,John L. Cameron,Scott E. Kern,Ralph H. Hruban
出处
期刊:PubMed
日期:2001-12-01
卷期号:7 (12): 3862-8
被引量:337
摘要
Effective new markers of pancreatic carcinoma are urgently needed. In a previous analysis of gene expression in pancreatic adenocarcinoma using serial analysis of gene expression (SAGE), we found that the tag for the mesothelin mRNA transcript was present in seven of eight SAGE libraries derived from pancreatic carcinomas but not in the two SAGE libraries derived from normal pancreatic duct epithelial cells. In this study, we evaluate the potential utility of mesothelin as a tumor marker for pancreatic adenocarcinoma.Mesothelin mRNA expression was evaluated in pancreatic adenocarcinomas using reverse-transcription PCR (RT-PCR) and in situ hybridization, whereas mesothelin protein expression was evaluated by immunohistochemistry.Using an online SAGE database (http://www.ncbi.nlm.gov/SAGE), we found the tag for mesothelin to be consistently present in the mesothelioma, ovarian cancer, and pancreatic cancer libraries but not in normal pancreas libraries. Mesothelin mRNA expression was confirmed by in situ hybridization in 4 of 4 resected primary pancreatic adenocarcinomas and by RT-PCR in 18 of 20 pancreatic cancer cell lines, whereas mesothelin protein expression was confirmed by immunohistochemistry in all 60 resected primary pancreatic adenocarcinomas studied. The adjacent normal pancreas in these 60 cases did not label, or at most only rare benign pancreatic ducts showed weak labeling for mesothelin.Mesothelin is a new marker for pancreatic adenocarcinoma identified by gene expression analysis. Mesothelin overexpression in pancreatic adenocarcinoma has potential diagnostic, imaging, and therapeutic implications.
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