Determination of Total Cholesterol in Serum by Gas Chromatography-Mass Spectrometry

In this study, a reliable protocol for determination of total cholesterol in serum using gas chromatography-mass spectrometry was established.The total free cholesterol was extracted from the serum with chloroform and derivatized with bis(trimethylsilyl)trifluoroacetamide, analyzed without the saponification step commonly used.Linear calibration curves of cholesterol were obtained from the concentration 0.1 to 15 mmol L -1 .The limit of detection was 0.04 mmol L -1 .Finally, the proposed method was to explore the influence of high cholesterol diet on total cholesterol in serum of rats.