Determination of polysorbate 80 in Reduning injection by HGPC-ELSD
色谱法
化学
色谱检测器
作者
Juan Shen,Qiao Zhang,Jiachun Li,Yu-An Bi,Zhen-Zhong Wang,Wei Xiao
出处
期刊:China journal of Chinese materia medica [China Journal of Chinese Materia Medica] 日期:2014-08-01被引量:1
标识
DOI:10.4268/cjcmm20141523
摘要
Objective To establish the method for determining polysorbate 80 in Reduning injection by HPLC-ELSD, and to control the mass of polysorbate 80 in Reduning injection. Method It was performed by HGPC-ELSD with TOSHTSK-GEL G4000PWxl (7.8 mm x 300 mm, 10 μm). Water was used as mobile phase, the flow rate was 0.7 mL x min(-1), and the temperature was set at 30°C. The evaporated light scattering detector was adopted. The drift tube temperature was 55°C, and nitrogen was used as carrier gas, with the flow rate of 2.0 L x min(-1) and gain of 1.0. Result The calibration curve showed good linearity of polysorbate 80 in the test range from 1.01 to 15.20 g x L(-1) (r2 = 0.999 3). The recovery rate was 98.10% with RSD of 2.0%. Conclusion The method is simple, rapid, accurate and reliable and suitable for the determination of polysorbate 80 in Reduning injection.