Zebrafishcox17modulates primitive erythropoiesis via regulation of mitochondrial metabolism to facilitate hypoxia tolerance

Abstract Cox17 is required in the assembly of mitochondrial intermembrane space (IMS) and Cu metallization of cytochrome C oxidase (CcO) in mitochondria as well as Cu homeostasis in cells. Cox deficiency is associated with hematopoietic diseases such as tubulopathy and leukodystrophy, but whether and how cox17 functions in hematopoiesis are still unknown. Here, we report the effects of zebrafish cox17 deficiency on primitive erythropoiesis, mitochondrial metabolism, and hypoxia tolerance. Cox17 −/− larvae were sensitive to hypoxia stress, with reduced primitive erythropoiesis. Meanwhile, cox17 −/− mutants showed a significant reduction in the expression of pivotal transcriptional regulators in erythropoiesis, such as scl , lmo2 , and gata1a at 14 h post fertilization (hpf), with expression remaining downregulated for scl but upregulated for lmo2 and gata1a at 24 hpf. Mechanistically, cox17 −/− mutants showed impaired mitochondrial metabolism, coupled with a significant decrease in the mitochondrial membrane potential, ATP and SAM content, and the ratio of SAM and SAH. Additionally, disrupting mitochondrial metabolism in wild type (WT) larvae treated with carbonyl cyanide 3‐chlorophenylhydrazone (CCCP) could mimic the primitive erythropoiesis defects observed in cox17 −/− mutants. Moreover, cox17 −/− mutants exhibited significantly downregulated WNT signaling and upregulated ER stress, with a significant reduction of beta‐Catenin in gata1a + cells and of binding enrichment in both scl and lmo2 promoters of the WNT transcriptional factor TCF4. This is the first report on the novel linkage of cox17 deficiency with defective primitive erythropoiesis and reduced hypoxia tolerance. This study has shed light on the potential mechanism by which Cox deficiency, especially cox17 deficiency, induces Cu homeostasis imbalance, leading to hematopoietic diseases.