细胞因子
生物
转录组
SOCS2
受体
趋化因子
细胞因子受体
细胞生物学
玉米赤霉烯酮
生殖系
免疫学
内分泌学
基因
真菌毒素
基因表达
遗传学
生物技术
抑制器
作者
Jingjing Wang,Hairui Tian,Hongyang Li,Jian Guo Wen,Ruihua Huang,Kang Zou,Liming Hou,Pinghua Li
标识
DOI:10.1016/j.theriogenology.2023.08.004
摘要
Zearalenone (ZEA) is a prevalent mycotoxin functions as an endocrine disrupter to the reproductive systems of farm animals, especially in pigs. To evaluate the effect and the underlying molecular changes that occurred when the porcine germline stem cells were exposed to ZEA, prospermatogonia (ProSGs) were enriched and treated with a gradient concentration (0-10 μM) of ZEA for 2-8 days. Our results showed that the ZEA treatment inhibited the proliferation of ProSGs in a dose-dependent manner with a critical concentration at 1 μM. Transcriptome analysis revealed that the differentially expressed genes mainly concentrated on the molecular function of positive regulation of response to stimulus, and the most enriching pathway is cytokine-cytokine receptor interaction. ZEA exposure decreased a buck of cytokine/chemokine expression involved in the inflammatory response and stem cells maintenance/self-renewal, moreover, some energy expenditure and anti-apoptosis genes were also down-regulated, while the up-regulated genes were mainly connected with the innate immunity. These data demonstrate that ZEA induces multiply cellular damage and may eventually do harm to the health and fertility of animals.
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