Efficient production of xylobiose and xylotriose from corncob by mixed acids and xylanase hydrolysis

Propionic acid (PA) hydrolysis offers a potential pathway for industrial xylooligosaccharide (XOS) production owing to efficiency and simplicity of the process. However, the cost of XOS production needs to be reduced as PA is expensive. This work proposed a strategy of mixed acids hydrolysis, replacing 20% of PA with formic acid (FA), and combined with xylanase hydrolysis to reduce production costs and increase the production of XOS from corncob. The hydrolysis of corncob using mixed FA and PA in a mass ratio of 2:8 produced 61.8% XOS. Xylanase hydrolysis of corncob residue improved XOS yield to 73.1%. Among them, the X2 + X3 yield was as high as 50.6%. Economic evaluation showed that the combined process reduced the XOS production cost by 10.8% compared to PA hydrolysis. The strategy of using FA instead of 20% PA for hydrolysis and enzymatic hydrolysis, with high XOS and monosaccharide yields from corncob, has potential industrial promise.