生物发生
分泌物
细胞外
免疫系统
细胞生物学
功能(生物学)
微泡
营养感应
化学
胞外囊泡
外体
谷氨酰胺
生物
生物化学
信号转导
免疫学
小RNA
氨基酸
基因
作者
Yizhuo Wang,Ping Lou,Yijing Xie,Shuyun Liu,Lan Li,Chengshi Wang,Dan Du,Younan Chen,Yanrong Liu,Jun Cheng,Jingping Liu
出处
期刊:Science Advances
[American Association for the Advancement of Science (AAAS)]
日期:2024-02-16
卷期号:10 (7)
标识
DOI:10.1126/sciadv.adj1290
摘要
Extracellular vesicle (EV)-based immunotherapeutics have emerged as promising strategy for treating diseases, and thus, a better understanding of the factors that regulate EV secretion and function can provide insights into developing advanced therapies. Here, we report that nutrient availability, even changes in individual nutrient components, may affect EV biogenesis and composition of immune cells [e.g., macrophages (Mφs)]. As a proof of concept, EVs from M1-Mφ under glutamine-depleted conditions (EVGLN-) had higher yields, functional compositions, and immunostimulatory potential than EVs from conventional GLN-present medium (EVGLN+). Mechanistically, the systemic metabolic rewiring (e.g., altered energy and redox metabolism) induced by GLN depletion resulted in up-regulated pathways related to EV biogenesis/cargo sorting (e.g., ESCRT) and immunostimulatory molecule production (e.g., NF-κB and STAT) in Mφs. This study highlights the importance of nutrient status in EV secretion and function, and optimizing metabolic states and/or integrating them with other engineering methods may advance the development of EV therapeutics.
科研通智能强力驱动
Strongly Powered by AbleSci AI