Improved Catalytic Performance of Lipase Within Hydrogel Microspheres Incorporating L/D‐Co3O4 Nanoparticles

Abstract Lipases have drawn significant attention due to their crucial roles across various industries, including oil processing, food, medicine, and daily chemicals. Nevertheless, their catalytic efficiency is susceptible to fluctuations in external environmental conditions, which remains a major barrier to their broader application. In this research, chiral hydrogel microspheres (HMSs) are developed by incorporating L/D‐Co 3 O 4 nanoparticles (NPs) into the synthetic process of HMSs, which are discovered to enhance the catalytic performance of lipases. The improved catalytic activity of lipases is observed to be influenced by the chiral environment of L/D‐Co 3 O 4 ‐HMSs. Specifically, when 2 wt.% lipases are encapsulated in L‐Co 3 O 4 ‐HMSs containing 10 wt.% L‐Co 3 O 4 NPs, their catalytic activity is 2.11 times higher than when encapsulated in D‐Co 3 O 4 ‐HMSs, resulting in a 2.62‐fold increase in catalytic activity compared to free lipase. Moreover, the L‐Lip‐HMS 3 , which contained 2 wt.% lipases and 10 wt.% L‐Co 3 O 4 NPs, exhibited superior inhibitory capability over D‐Lip‐HMS 3 in differentiating fibroblast mouse cells into adipocytes, owing to its enhanced catalytic capabilities. This study not only highlights the importance of chirality in improving the catalytic performance of natural enzymes but also provides a new perspective on developing catalytic systems that can endow natural enzymes with higher catalytic performances.