Evaluation of Neural, Systemic and Extracerebral Activations During Active Walking Tasks in Older Adults using fNIRS

Functional near infrared spectroscopy (fNIRS) is being increasingly used to assess brain hemodynamic responses during active walking in older adults due to its wearability, and relative immunity to motion artifacts. Specifically, fNIRS allows for continuous monitoring of brain activations that vary in response to experimental manipulations of cognitive demands during active walking tasks. Studies using fNIRS highlighted increased involvement of the prefrontal cortex (PFC) in dual compared to single task walking, operationalized using oxygenated hemoglobin (HbO), due to increasing attention demands inherent in the former task condition in aging and clinical populations. However, current literature utilizing fNIRS in mobility research has not been uniform in terms of fNIRS instrumentation characteristics and the accompanying signal processing methods to separate various signal sources (i.e. neural activations, extracerebral signals, systemic responses) which can raise questions about prior research findings. In our previous studies, we have used a forehead fNIR sensor (fNIR Imager 1100 by fNIR Devices, LLC) with 2.5 cm source detector separation (SDS) at 2 Hz sampling rate which allowed us to reliably evaluate changes in brain activations in the PFC during active walking. However, there exists other fNIRS devices incorporating a number of different types of light sources and detectors allowing multiple channels of long (3 cm SDS) and short (0.8 cm SDS) distance measurements in complex configurations for the monitoring of cognitive activations on various head locations at different depths with higher sampling rates of ~5 Hz (i.e. NIRx sensor, NIR Sport2 by NIRx Medizintechnik GmbH). Such involved designs further allowed the implementation of advanced signal processing algorithms to separate and evaluate neural, systemic and extracerebral signal contributions on the overall measurements. In this study, we collected brain imaging data on a sample of healthy older adults (n =15, age ) under single (STW) and dual task walking (DTW) conditions; participants were evaluated twice during one study visit, once wearing fNIR sensor and a second time while wearing NIRx sensor. This study design allowed us to address critical gaps in the extant literature concerning fNIRS-derived brain activations during active walking. Specifically, we evaluated potential effects of penetration depth as defined by the SDS of the fNIRS device, extracerebral activations (i.e. skin blood flow) and systemic signals (i.e. heart rate) on the observed HbO increases from STW to DTW. Our findings suggested that PFC activation differences between STW and DTW conditions observed in older adults were consistent across fNIRS instrumentations and the observed differences in HbO between STW and DTW were not materially influenced by scalp activations or systemic changes. Nevertheless, efforts to optimize extraction of fNIRS-derived brain signal measurements should continue taking advantage of technological advancement.