相对标准差
标准差
变异系数
稳健性(进化)
摩尔吸收率
绝对偏差
计算生物学
化学
色谱法
生物系统
统计
数学
生物
生物化学
检出限
物理
光学
基因
作者
Yan Xu,Zhongcai Shao,Lei Zhang,Yuyan Cao,Zhuoyu Liu,Zhongli Zhang
标识
DOI:10.1016/j.ab.2024.115508
摘要
Biologicals developers often face challenges in accurately determining the extinction coefficient (EC) measurement. We have successfully improved the precision and robustness of the widely recognized amino acid analysis method for EC determination, through a stepwise optimization process. Extensive analyses based on 114 observations, covering eight proteins over three years were performed, with a maximum relative standard deviation of 1.5% among multiple analysts, and a maximum deviation of 2.8% from the theoretical EC across the eight given proteins examined.
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