DNA旋转酶
去酰胺
行动方式
毒素
DNA超螺旋
化学
生物化学
DNA
天冬酰胺
质粒
生物
大肠杆菌
酶
基因
DNA复制
作者
William M. Parks,Andrew R. Bottrill,Olivier A. Pierrat,Marcus C. Durrant,Anthony Maxwell
出处
期刊:Biochimie
[Elsevier]
日期:2007-04-01
卷期号:89 (4): 500-507
被引量:62
标识
DOI:10.1016/j.biochi.2006.12.005
摘要
Microcin B17 (MccB17) is a peptide-based bacterial toxin that targets DNA gyrase, the bacterial enzyme that introduces supercoils into DNA. The site and mode of action of MccB17 on gyrase are unclear. We review what is currently known about MccB17-gyrase interactions and summarise approaches to understanding its mode of action that involve modification of the toxin. We describe experiments in which treatment of the toxin at high pH leads to the deamidation of two asparagine residues to aspartates. The modified toxin was found to be inactive in vivo and in vitro, suggesting that the Asn residues are essential for activity. Following on from these studies we have used molecular modelling to suggest a 3D structure for microcin B17. We discuss the implications of this model for MccB17 action and investigate the possibility that it binds metal ions.
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