医学
磁共振成像
冲程(发动机)
大脑中动脉
病变
梗塞
磁共振血管造影
蛛网膜下腔出血
闭塞
缺血
病态的
血管闭塞
放射科
麻醉
内科学
外科
机械工程
心肌梗塞
工程类
作者
Tibo Gerriets,Erwin Stolz,Maureen Walberer,Clemens Müller,Carina Röttger,Alexander Kluge,Manfred Kaps,Marc Fisher,Georg Bachmann
出处
期刊:Stroke
[Ovid Technologies (Wolters Kluwer)]
日期:2004-10-01
卷期号:35 (10): 2372-2377
被引量:93
标识
DOI:10.1161/01.str.0000142134.37512.a7
摘要
Investigating focal cerebral ischemia requires animal models that are relevant to human stroke. Complications and side effects are common among these models. The present study describes potential pitfalls in 3 techniques for middle cerebral artery occlusion (MCAO) in rats using magnetic resonance imaging (MRI) and magnetic resonance angiography (MRA).Rats were subjected to temporary MCAO for 90 minutes using the suture technique (group I; n=10) or to permanent MCAO using the suture technique (group II; n=10) or the macrosphere technique (group III; n=10). Clinical evaluation was performed after 3 hours and 24 hours. After 24 hours, animals underwent MRI and MRA to determine lesion size and the intracranial vascular status.Hemispheric lesion volume was significantly smaller in group I (14.6%) compared with groups II (35.2%; P<0.01) and III (21.3%; P<0.05). Two animals (1 each in group II and III) did not demonstrate neurological deficits and had no lesion on MRI and a patent MCA main stem on MRA. Subarachnoid hemorrhage was detected in 2 animals (1 each in group I and II). MRA indicated a patent MCA main stem in 2 animals (group II), although both rats displayed neurological deficits. Hypothalamic infarction with subsequent pathological hyperthermia was detected in all animals in group II and in 1 rat in group III.Model failures occurred frequently in all groups. MRI and MRA helps to identify animals that need to be excluded from experimental stroke studies.
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