醌
量子点
氧化还原
猝灭(荧光)
化学
电子转移
对苯二酚
多巴胺
光化学
电子受体
接受者
生物传感器
纳米技术
荧光
材料科学
无机化学
有机化学
生物化学
神经科学
物理
生物
量子力学
凝聚态物理
作者
Igor L. Medintz,Michael H. Stewart,Scott A. Trammell,Kimihiro Susumu,James B. Delehanty,Bingchu Mei,Joseph S. Melinger,Juan B. Blanco‐Canosa,Philip E. Dawson,Hedi Mattoussi
出处
期刊:Nature Materials
[Springer Nature]
日期:2010-08-01
卷期号:9 (8): 676-684
被引量:430
摘要
The use of semiconductor quantum dots (QDs) for bioimaging and sensing has progressively matured over the past decade. QDs are highly sensitive to charge-transfer processes, which can alter their optical properties. Here, we demonstrate that QD-dopamine-peptide bioconjugates can function as charge-transfer coupled pH sensors. Dopamine is normally characterized by two intrinsic redox properties: a Nernstian dependence of formal potential on pH and oxidation of hydroquinone to quinone by O(2) at basic pH. We show that the latter quinone can function as an electron acceptor quenching QD photoluminescence in a manner that depends directly on pH. We characterize the pH-dependent QD quenching using both electrochemistry and spectroscopy. QD-dopamine conjugates were also used as pH sensors that measured changes in cytoplasmic pH as cells underwent drug-induced alkalosis. A detailed mechanism describing the QD quenching processes that is consistent with dopamine's inherent redox chemistry is presented.
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