Immunodiagnosis of bovine fasciolosis using Fasciola hepatica excretory-secretory antigens ELISA.

Two hundreds blood samples and the corresponding faecal and/or bile (114 Egyptian buffaloes, 68 native breed cattle and 18 imported cattle) were examined for Fasciola infection. ELISA revealed that 8 (7.00%) buffaloes, 52 (76.50%) native breed cattle and 15 (83.33%) imported cattle were positive. Parasitologically, the results revealed that 38 (33.33%) buffaloes and 18 (26.50%) native breed cattle were positive, while all imported cattle were free from Fasciola. So, ELISA technique using F. hepatica excretory-secretory (E/S) antigens is of diagnostic value for cattle fasciolosis rather than for buffaloes.