Determination of Polysaccharide in Rhizoma Polygonatii by Phenol-Sulfuric Acid and DNS Methods

Objective:To establish a simple determination method of polysaccharide in Rhizoma Polygonatii,which is not influenced by the content of monosaccharide.Method:The total sugar was determined by phenol-sulfuric acid method while the concentration of reducing sugar was determined by 3,5-dinitrosalicylic acid(DNS) method,the contentration of polysaccharide=Ct(the concentration of total sugar)-Cs(the concentration of reducing sugar).Result:It was used to evaluate the content of polysaccharide collected in different area,the concentrations of polysaccharide in Polygonatum Rhizoma collected fromYunnan province are relatively higher.Conclusion:The results showed above methods are convenient,stable and accurate,which can be used for quality evaluation of Polygonatum Rhizoma and its preparations.