Objective To determine the content of polysaccharides in Houttuynia cordata from various cultivation locations.Methods The contents of polysaccharide were measured by phenol-H2SO4 colorimetry.Results The contents of polysaccharides in Houttuynia cordata from various cultivation locations varied from 15.73%~ 20.29%.Conclusion There is great difference in polysaccharide content in Houttuynia cordata from various cultivation locations.