Inhibitory effects of c-myc antisense oligonucleotides on the proliferation of MKN-45 cells

Objective To observe the inhibitory effects of c-myc antisense oligonucleotides on the proliferation of MKN-45 cells.Methods Cell proliferation was measured by MTT assay.Expres- sion of c-myc protein was measured by immunocytochemistry after transfection.Apoptosis of cells was detected by flow cytometry and fluorescence microscope.Results After sealing c-myc gene with ASODN,the proliferation of MKN-45 cells was inhibited markedly.FCM analysis showed that an obvious apoptosis peak appeared after transfection.The immunocytochemistry staining showed that c-myc protein expression was suppressed significantly 64.9%±5.68%.Conclusion Liposome-mediated c-myc ASODN can evidently inhibit cell proliferation,down-regulate c-myc protein expression,and induce apoptosis of MKN-45 cells.