纳米探针
树枝状大分子
胶体金
胶质瘤
化学
配体(生物化学)
材料科学
PEG比率
纳米颗粒
生物物理学
纳米技术
癌症研究
受体
医学
生物化学
生物
财务
经济
作者
Yujie Li,Lei Zhao,Xiaoying Xu,Na Sun,Wei Qiao,Yan Xing,Mingwu Shen,Meilin Zhu,Xiangyang Shi,Jinhua Zhao
标识
DOI:10.1166/jbn.2019.2760
摘要
Chemokine receptor-4 (CXCR4) has been demonstrated to be overexpressed in glioma cells and associated with poor survival. In the present study, we designed and synthesized 99mTc-labeled low-generation dendrimer-entrapped gold nanoparticles (Au DENPs), which were modified with CXCR4-ligand for targeted single photon emission computed tomography (SPECT)/computed tomography (CT) imaging of glioma. Generation two poly(amidoamine) dendrimers were sequentially conjugated with diethylenetriamine pentacetate acid (DTPA) and polyethylene glycol (PEG) modified with FC131 peptide (a CXCR4-specific ligand), and were then utilized as a template for entrapment of gold nanoparticles (Au NPs). After radiolabeling with 99mTc via DTPA chelation, the glioma-targeting SPECT/CT dual mode nanoprobe {(Au0)6-G2-99m-DTPA-(PEG-FC131)} DENPs were generated. The FC131-functionalized Au DENPs before and after 99mTc radiolabeling were well characterized. The results revealed that prior to 99mTc radiolabeling, FC131-functionalized Au DENPs had a uniform size distribution, acceptable biocompatibility in the given Au concentration range and could specifically target glioma cells due to the attached FC131. Furthermore, the FC131-functionalized Au DENPs could be readily radiolabeled by 99m Tc with good radiochemical stability and high radiochemical purity. The {(Au0)6-G2-99mTc-DTPA-(PEG-FC131)} DENPs appeared to be effective in targeted SPECT/CT imaging of glioma cells in vitro and in vivo in a subcutaneous tumor model. The developed FC131-functionalized dendrimer-based nanoprobe may hold a great potential for utilization in SPECT/CT imaging of different CXCR4-overexpressing cancers.
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