原肠化
生物
原始条纹
中胚层
内胚层
胚胎干细胞
器官发生
细胞分化
细胞生物学
诱导多能干细胞
遗传学
发育生物学
基因表达谱
胚胎
计算生物学
基因
外胚层
外胚层
基因表达
作者
Blanca Pijuan-Sala,J. B. Griffiths,Carolina Guibentif,Tom W. Hiscock,Wajid Jawaid,Fernando J. Calero-Nieto,Carla Mulas,Ximena Ibarra-Soria,Richard Tyser,Debbie Ho,Wolf Reik,Shankar Srinivas,Benjamin D. Simons,Jennifer Nichols,John C. Marioni,Berthold Göttgens
出处
期刊:Nature
[Springer Nature]
日期:2019-02-01
卷期号:566 (7745): 490-495
被引量:672
标识
DOI:10.1038/s41586-019-0933-9
摘要
Across the animal kingdom, gastrulation represents a key developmental event during which embryonic pluripotent cells diversify into lineage-specific precursors that will generate the adult organism. Here we report the transcriptional profiles of 116,312 single cells from mouse embryos collected at nine sequential time points ranging from 6.5 to 8.5 days post-fertilization. We construct a molecular map of cellular differentiation from pluripotency towards all major embryonic lineages, and explore the complex events involved in the convergence of visceral and primitive streak-derived endoderm. Furthermore, we use single-cell profiling to show that Tal1−/− chimeric embryos display defects in early mesoderm diversification, and we thus demonstrate how combining temporal and transcriptional information can illuminate gene function. Together, this comprehensive delineation of mammalian cell differentiation trajectories in vivo represents a baseline for understanding the effects of gene mutations during development, as well as a roadmap for the optimization of in vitro differentiation protocols for regenerative medicine. Single-cell profiling is used to create a molecular-level atlas of cell differentiation trajectories during gastrulation and early organogenesis in the mouse.
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