核酸
核糖核酸
环介导等温扩增
逆转录酶
严重急性呼吸综合征冠状病毒2型(SARS-CoV-2)
酶
重组酶聚合酶扩增
2019年冠状病毒病(COVID-19)
计算生物学
多重位移放大
分子生物学
化学
DNA
病毒学
生物
聚合酶链反应
生物化学
基因
医学
病理
传染病(医学专业)
DNA提取
疾病
作者
Mohsen Mohammadniaei,Ming Zhang,Jon Ashley,Ulf Bech Christensen,Lennart Friis‐Hansen,Rasmus Gregersen,Jan Gorm Lisby,Thomas Benfield,Finn Erland Nielsen,Jens Henning Rasmussen,Elisabeth A. Pedersen,Anne Christine Rye Olinger,Lærke Tørring Kolding,Maryam Naseri,Zheng Tao,Wentao Wang,Jan Gorodkin,Yi Sun
标识
DOI:10.1038/s41467-021-25387-9
摘要
Abstract The current nucleic acid signal amplification methods for SARS-CoV-2 RNA detection heavily rely on the functions of biological enzymes which imposes stringent transportation and storage conditions, high cost and global supply shortages. Here, a non-enzymatic whole genome detection method based on a simple isothermal signal amplification approach is developed for rapid detection of SARS-CoV-2 RNA and potentially any types of nucleic acids regardless of their size. The assay, termed non-enzymatic isothermal strand displacement and amplification (NISDA), is able to quantify 10 RNA copies.µL −1 . In 164 clinical oropharyngeal RNA samples, NISDA assay is 100 % specific, and it is 96.77% and 100% sensitive when setting up in the laboratory and hospital, respectively. The NISDA assay does not require RNA reverse-transcription step and is fast (<30 min), affordable, highly robust at room temperature (>1 month), isothermal (42 °C) and user-friendly, making it an excellent assay for broad-based testing.
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