清脆的
基因组编辑
Cas9
斑马鱼
计算生物学
生物
基因
基因组
功能(生物学)
遗传学
作者
Preeti Sharma,B Sharan Sharma,Ramtej J. Verma
出处
期刊:Progress in Molecular Biology and Translational Science
日期:2021-01-01
卷期号:: 69-84
被引量:12
标识
DOI:10.1016/bs.pmbts.2021.01.005
摘要
CRISPR/Cas9, once discovered as an adaptive immune system in bacteria, has emerged as a disruptive technology in the field of genetic engineering. Technological advancements in the recent past has enhanced the applicability of CRISPR/Cas9 tool for gene editing, gene therapies, developmental studies and mutational analysis in various model organisms. Zebrafish, one of the excellent animal models, is preferred for conducting CRISPR/Cas9 studies to assess the functional implication of specific genes of interest. CRISPR/Cas9 mediated gene editing techniques, such as, knock-out and knock-in approaches, provide evidences to identify the role of different genes through loss-of-function studies. Also, CRISPR/Cas9 has been proved to be an efficient tool for designing disease models for gene expression studies based on phenotypic screening. The present chapter provides an overview of CRISPR/Cas9 mechanism, different strategies for DNA modifications and gene function analysis, highlighting the translational applications for future prospects, such as screening of drug toxicity and efficacy.
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