细胞外基质
真皮
收缩(语法)
材料科学
生物医学工程
伤口愈合
巨噬细胞极化
血管生成
细胞生物学
新生血管
巨噬细胞
生物物理学
化学
解剖
外科
癌症研究
医学
生物
生物化学
体外
内科学
作者
Lei Chen,Zhiyong Li,Yongtai Zheng,Fei Zhou,Jingling Zhao,Qiyi Zhai,Zhaoqiang Zhang,Tianrun Liu,Yongming Chen,Shunan Qi
标识
DOI:10.1016/j.bioactmat.2021.09.008
摘要
Scar contraction frequently happens in patients with deep burn injuries. Hitherto, porcine dermal extracellular matrix (dECM) has supplied microenvironments that assist in wound healing but fail to inhibit scar contraction. To overcome this drawback, we integrate dECM into three-dimensional (3D)-printed dermal analogues (PDA) to prevent scar contraction. We have developed thermally gelled, non-rheologically modified dECM powder (dECMp) inks and successfully transformed them into PDA that was endowed with a micron-scale spatial structure. The optimal crosslinked PDA exhibited desired structure, good mechanical properties as well as excellent biocompatibility. Moreover, in vivo experiments demonstrated that PDA could significantly reduced scar contraction and improved cosmetic upshots of split thickness skin grafts (STSG) than the commercially available dermal templates and STSG along. The PDA has also induced an early, intense neovascularization, and evoked a type-2-like immune response. PDA's superior beneficial effects may attribute to their desired porous structure, the well-balanced physicochemical properties, and the preserved dermis-specific ECM cues, which collectively modulated the expression of genes such as Wnt11, ATF3, and IL1β, and influenced the crucial endogenous signalling pathways. The findings of this study suggest that PDA is a clinical translatable material that possess high potential in reducing scar contraction.
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