谷氨酸棒杆菌
生物量(生态学)
木质纤维素生物量
生物反应器
醋酸
乙酸钠
化学
产量(工程)
碳纤维
基质(水族馆)
碳源
制浆造纸工业
生物技术
食品科学
色谱法
生物化学
有机化学
发酵
材料科学
生物
农学
复合材料
工程类
复合数
冶金
基因
生态学
作者
Dirk Kiefer,Manuel Merkel,Lars Lilge,Rudolf Hausmann,Marius Henkel
标识
DOI:10.1016/j.biortech.2021.125666
摘要
Acetate represents a promising alternative carbon source for future industrial biotechnology. In this study, the high potential of Corynebacterium glutamicum for utilizing acetate as sole carbon source was demonstrated. Batch culture studies revealed that C. glutamicum ATCC 13032 naturally exhibits high acetate tolerance with maximum growth rates (µmax = 0.47 h−1) similar to those on D-glucose. Based on a simple and auto-regulated pH-coupled feeding strategy which utilizes bio-acetic acid in pure form, a novel and high-efficient fed-batch process was developed in a 42 L stirred-tank bioreactor. By optimizing the carbon-to-nitrogen (C/N) feeding ratio, maximum biomass concentrations of 80.2 gCDW/L were achieved with a space–time yield of 66.6 gCDW/L·d. In addition, a process model was implemented describing the time-courses of biomass growth and substrate concentrations. This is the first study in which an industrial platform organism was grown to high cell densities using green, lignocellulosic acetate as an alternative carbon source.
科研通智能强力驱动
Strongly Powered by AbleSci AI