谷胱甘肽
纳米传感器
化学
细胞内
荧光
癌细胞
荧光寿命成像显微镜
生物物理学
癌症
生物化学
组合化学
纳米技术
酶
量子力学
医学
生物
物理
内科学
材料科学
作者
Chunrong Li,Yuzhi Xu,Siyang Liu,Yanfei Zhang,Wen Yin,Zong Dai,Xiaoyong Zou
出处
期刊:Talanta
[Elsevier]
日期:2021-11-01
卷期号:234: 122650-122650
被引量:5
标识
DOI:10.1016/j.talanta.2021.122650
摘要
Ascorbic acid (AA) and glutathione (GSH), the most abundant intracellular reductive substances, have been widely used as biomarkers for cancer cells identification. The current methods relying on imaging of AA or GSH alone to identify cancer cells may cause systematic errors, since a mutual conversion relationship exists between AA and GSH. In this work, we propose a fluorescent nanosensor for the simultaneous imaging of intracellular reductive substances including AA and GSH. Biocompatible fluorescent silicon nanoparticles (SiNPs) with rich surface amine and carboxyl groups were synthesized. The fluorescence of the SiNP was initially quenched by chelation of Fe3+ ions, forming SiNP/Fe3+ complex as the fluorescent nanosensor. Upon the redox reaction with reductive substances, the nanosensor showed sensitively fluorescent recovery. Moreover, benefited from the efficient cellular uptake of the SiNP/Fe3+ and the overexpressed intracellular reductive substances in cancer cells, the fluorescent nanosensor was used to accurately identify the human breast carcinoma (MCF-7) cells from normal mammary epithelial (MCF-10A) cells by imaging of intracellular AA and GSH simultaneously. This strategy would be promising in imaging-guided precision cancer diagnosis.
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