代谢组学
异核分子
核磁共振波谱
代谢物
异核单量子相干光谱
质子核磁共振
二维核磁共振波谱
化学
计算生物学
核磁共振
生物
生物化学
色谱法
物理
作者
Jun Kikuchi,Takashi Hirayama
出处
期刊:Methods in molecular biology
日期:2007-01-01
卷期号:: 273-286
被引量:43
标识
DOI:10.1007/978-1-59745-244-1_15
摘要
Analytical methods for probing plant metabolism are taking on new significance in the era of functional genomics, metabolomics, and systems biology. Nuclear magnetic resonance (NMR) is becoming a key technology in plant metabolomics. Stable isotope labeling of cultured cells and higher organisms has been especially promising in that it allows the use of advanced heteronuclear NMR methodologies through a combination of in vivo and in vitro measurements. This new approach provides much better resolution of the metabolite mixture signals in the multidimensional NMR spectra than does the conventional one-dimensional 1H-NMR previously used in plant metabolomics. In this chapter, we describe the practical aspects of two key NMR technologies: uniform stable labeling of plants and in vitro heteronuclear NMR.
科研通智能强力驱动
Strongly Powered by AbleSci AI