神经干细胞
生物
神经细胞
祖细胞
干细胞
移植
人口
神经科学
细胞
细胞生物学
遗传学
医学
内科学
环境卫生
出处
期刊:Methods in molecular biology
日期:2013-01-01
卷期号:: 95-106
被引量:11
标识
DOI:10.1007/978-1-62703-574-3_9
摘要
The renewable source of neural stem cells (NSCs) with multi-lineage differentiation capability towards neurons, astrocytes, and oligodendrocytes represent an ideal supply for cell therapy of central nervous system (CNS) diseases. In spite of this, the clinical use of NSCs is hampered by heterogeneity, poor neuronal cell yield, predominant astrocytic differentiation of NSC progeny and possible uncontrolled proliferation, and tumor formation upon transplantation. The ability to generate highly enriched and defined neural cell populations from the renewable source of NSCs might overcome many of these impediments and pave the way towards their successful clinical applications. Here, we describe a simple method for NSC differentiation and subsequent purification of neuronal progenitor cells, taking advantage of size and granularity differences between neuronal cells and other NSC progeny. This highly enriched neuronal cell population provides an invaluable source of cells for both in vitro and in vivo studies.
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