H3K4me3
基因敲除
生物
基因
表观遗传学
DNA甲基化
RNA干扰
水稻
遗传学
组蛋白甲基化
组蛋白
甲基化
表型
基因表达
发起人
核糖核酸
作者
Wenxuan Lia,Yingying Hana,Feng Taoa,Kang Chonga
摘要
S-Adenosyl-l-methionine synthetase (SAMS) [EC 2.5.1.6] catalyzes to produce SAM (S-adenosyl-lmethionine), a universal methyl group donor in biochemical reactions in cells. However, less is known how SAMS controls plant development. Here, we demonstrate that OsSAMS1, 2 and 3 are essential for histone H3K4me3 and DNA methylation to regulate gene expression related to flowering in Oryza sativa. RNA interference (RNAi) transgenic rice with downregulated transcripts of OsSAMS1, 2 and 3 showed pleiotropic phenotypes, including dwarfism, reduced fertility, delayed germination, as well as late flowering. Delayed germination was largely rescued by application of SAM in the knockdown lines. Knockdown of OsSAMS1, 2 and 3 led to distinguished late flowering and greatly reduced the expression of the flowering key genes, Early heading date 1 (Ehd1), Hd3a and RFT1 (rice FT-like genes). Moreover, the histone H3K4me3 and symmetric DNA methylation at these genes were greatly reduced. Thus, SAM deficiency suppressing DNA and H3K4me3 transmethylations at flowering key genes led to a late-flowering phenotype in rice. This information could help elucidate the mechanism of epigenetic control flowering transition. © 2011 Elsevier GmbH. All rights reserved.
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