The IVF Cycle to Come: Laboratory Innovations

In the first reported case of IVF and embryo development in the human, Rock and Menkin made the following description of the culture and development of a fertilized embryo: “The eggs were incubated in serum for 22.5 hours, being washed in salt solution before and after incubation, and then exposed to a washed sperm suspension in Locke’s solution for two hours at room temperature. They were again washed in Locke’s solution and cultured in fresh serum for 45 hours.