Application progress of in vitro lung ischemia/reperfusion injury models

Background Hypoxia/reoxygenation (H/R) injury of alveolar epithelial cells (AECs) or pulmonary microvascular endothelial cells (PMECs) is an in vitro simulated model for studying the mechanisms and protective strategies of lung ischemia/reperfusion injury (LI/RI).Objective To explore the methods of establishing in vitro lung ischemia/reperfusion injury,models and related modeling issues,and to provide reference for effective:nodeling.Content Review of the research and application of lung iscbenfia/reperfusion injury models,including the general procedure of modeling,cell category and selection,methods of H/R,evaluation parameters of injury,etc.Trend There is no a uniform method to model pulmonary cell H/R injury so far.further research is needed. Key words: Alveolar epithelial cells;  Pulmonary microvascular endothelial cells;  Hypoxia/Reoxygenation;  Lung ischemia/reperfusion injury