Bioderivatization as a concept for renewable production of chemicals that are toxic or poorly soluble in the liquid phase

Significance Microorganisms can be rationally engineered to convert CO 2 and H 2 O into chemicals, replacing those made from fossil fuels today. Sometimes such chemicals are poorly soluble in water or negatively affect the growth of the microorganism, resulting in cost-inefficient manufacturing. In nature, this problem is often solved by converting incompatible chemicals into those more compatible with the host and/or environment. Inspired by this, we propose a similar strategy for engineered biotechnology, whereby biochemical conversion inside the microorganism is followed by chemical reversal once outside. The principle was demonstrated with 1-octanol by implementing two different conversion methods in two different species, showing enhanced bioproduction in most cases. The approach may stimulate commercialization of sustainable and renewable production of chemicals.