生物
核受体
酶诱导剂
二烯丙基二硫化物
基因
内分泌学
分子生物学
化学
酶
内科学
生物化学
医学
转录因子
细胞凋亡
作者
Tatsuya Sueyoshi,William D. Green,Kellie Vinal,Tyler S. Woodrum,Rick Moore,Masahiko Negishi
出处
期刊:PLOS ONE
[Public Library of Science]
日期:2011-06-15
卷期号:6 (6): e21229-e21229
被引量:34
标识
DOI:10.1371/journal.pone.0021229
摘要
Constituent chemicals in garlic extract are known to induce phase I and phase II enzymes in rodent livers. Here we have utilized Car+/+ and Car−/− mice to demonstrate that the nuclear xenobiotic receptor CAR regulated the induction of the estrogen sulfotransferase Sult1e1 gene by diallyl sulfide (DAS) treatment in mouse liver. DAS treatment caused CAR accumulation in the nucleus, resulting in a remarkable increase of SULT1E1 mRNA (3,200 fold) and protein in the livers of Car+/+ females but not of Car−/− female mice. DAS also induced other CAR-regulated genes such as Cyp2b10, Cyp3a11 and Gadd45β. Compared with the rapid increase of these mRNA levels, which began as early as 6 hourrs after DAS treatment, the levels of SULT1E1 mRNA began increasing after 24 hours. This slow response to DAS suggested that CAR required an additional factor to activate the Sult1e1 gene or that this activation was indirect. Despite the remarkable induction of SULT1E1, there was no decrease in the serum levels of endogenous E2 or increase of estrone sulfate while the clearance of exogenously administrated E2 was accelerated in DAS treated mice.
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