生物相容性
细胞包封
材料科学
马来酰亚胺
牛血清白蛋白
自愈水凝胶
肽
点击化学
再生医学
纳米技术
高分子化学
细胞
化学
色谱法
生物化学
冶金
作者
Yin Chen,Xin Dai,Lu Huang,Yong Sun,Ho Nam Chan,Bo Shen,Xiaoqian Zeng,Zhenguo Wu,I‐Ming Hsing,Zhihong Guo,Hongkai Wu
标识
DOI:10.1002/adfm.201502942
摘要
A universal and facile approach to modifying proteins so that they can rapidly form hydrogel upon mixing with crosslinkers is presented. The concept of it is to introduce maleimide, which is highly reactive with dithiol‐containing crosslinkers via thiol‐ene click chemistry, onto proteins. Bovine serum albumin (BSA) is used as a model protein due to its good stability and low cost. The results here show that a protein hydrogel can be readily formed by blending modified BSA and resilin‐related peptide crosslinker solutions at a proper ratio. The hydrogel exhibits good elasticity and tunable mechanical as well as biochemical properties. Moreover, it allows convenient 3D cell encapsulation and shows good biocompatibility. Muscle cells embedded in the hydrogel are promoted to spread by incorporating arginyl‐glycyl‐aspartic acid (RGD)‐containing peptide into the system, thus warranting a bright future of it in regenerative medicine.
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