胡杨
生物
基因
拟南芥
转基因
拟南芥
互补DNA
调节器
细胞生物学
耐旱性
转基因作物
功能(生物学)
基因表达
遗传学
植物
突变体
作者
Jinhuan Chen,Dongzhi Zhang,Chong Zhang,Xinli Xia,Weilun Yin,Qianqian Tian
出处
期刊:PLOS ONE
[Public Library of Science]
日期:2015-10-02
卷期号:10 (10): e0139466-e0139466
被引量:37
标识
DOI:10.1371/journal.pone.0139466
摘要
A PP2C homolog gene was cloned from the drought-treated cDNA library of Populus euphratica. Multiple sequence alignment analysis suggested that the gene is a potential ortholog of HAB1. The expression of this HAB1 ortholog (PeHAB1) was markedly induced by drought and moderately induced by ABA. To characterize its function in ABA signaling, we generated transgenic Arabidopsis thaliana plants overexpressing this gene. Transgenic lines exhibited reduced responses to exogenous ABA and reduced tolerance to drought compared to wide-type lines. Yeast two-hybrid analyses indicated that PeHAB1 could interact with the ABA receptor PYL4 in an ABA-independent manner. Taken together; these results indicated that PeHAB1 is a new negative regulator of ABA responses in poplar.
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