芍药苷
根(腹足类)
色谱法
化学
甘草苷
肉桂醛
梯度洗脱
甘草甜素
高效液相色谱法
大黄素
阿魏酸
肉桂酸
药理学
医学
有机化学
催化作用
生物
植物
生物化学
作者
Horng Liang Lay,Chia Chi Chen,Shiow Chyn Huang,Thau Ming Cham,Tian Shung Wu
标识
DOI:10.38212/2224-6614.2540
摘要
A facile HPLC method for the reduction and quantitative measurement of ten marker substances, the active ingredients in formula preparation of Wan-Yin-Gau, was established under the gradient elution in the reversed-phase mode. These marker substances included cinnamic acid, cinnamaldehyde (Cinnamomi Cortex), isoimperatorin (Angelicae Dahuricae Radix and Notopterygii Rhizoma), ferulic acid (Angelicae Sinensis Radix), paeoniflorin (Paeoniae Radix), glycyrrhizin (Glycyrrhizae Radix), harpagoside (Scrophulariae Radix), emodin, sennoside A, and sennoside B (Rhei Rhizoma). The ingredients in the water-based and oil-based patches from different manufactures were also analyzed for quality evaluation. Extracted samples were analyzed with reverse-phase column (Inertsil 5 ODS-2, 4.6 i.d. × 250 mm) at 30°C, eluted with a mixture of 20% and 70% acetonitrile aqueous solution in gradient manner at a flow rate of 1.0 mL/min, and detected at 250 nm. Relative coefficients of variation of intra- and inter-day analysis were less than 5%. All the recoveries were 96.29-103.46%. This method could be applied for the simultaneous determination of ten marker substances in "Wan-Yin-Gau".
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