化学
色谱法
丙基硫氧嘧啶
硫氧嘧啶
检出限
尿
液相色谱-质谱法
串联质谱法
高效液相色谱法
质谱法
甲状腺
内科学
生物化学
激素
医学
作者
Madis Lõhmus,Kalle Kallaste,Bruno Le Bizec
标识
DOI:10.1016/j.chroma.2009.04.005
摘要
Thyreostatic compounds could be illegally administered to animals in order to obtain a weight gain due to a higher retention of water in the edible tissue and the gastro-intestinal tract. In the European Union their use for animal production is banned since 1981. Recently a highly sensitive method exploiting the determination of thyreostats with 3-iodobenzylbromide prior to purification to determine thyreostats in urine and other matrices was reported. For the first time, the UPLC instrumentation was used to separate the 3-iodobenzyl derivatives of various thyreostats. The deuterated internal standards tapazole-d3 and propylthiouracil-d5 were for the first time used for the quantification of tapazole, thiouracil, methylthiouracil, propylthiouracil, phenylthiouracil and mercaptobenzimidazole. The confirmative quantitative liquid chromatographic tandem mass spectrometric (LC–MS/MS) method was validated according to Commission Decision 2002/657/EC. The decision limit (CCα) and the detection capability (CCβ) were found to be for all compounds below the recommended value of 10 μg kg−1.
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