外胚层
生物
同源盒蛋白纳米
细胞生物学
胚泡
滋养层
胚胎干细胞
纳米同源盒蛋白
胚胎
诱导多能干细胞
遗传学
原肠化
胚胎发生
基因
怀孕
胎儿
胎盘
作者
Mila Roode,Kathryn Blair,Philip Snell,Kay Elder,Sally Marchant,Austin Smith,Jennifer Nichols
标识
DOI:10.1016/j.ydbio.2011.10.030
摘要
Mouse embryos segregate three different lineages during preimplantation development: trophoblast, epiblast and hypoblast. These differentiation processes are associated with restricted expression of key transcription factors (Cdx2, Oct4, Nanog and Gata6). The mechanisms of segregation have been extensively studied in the mouse, but are not as well characterised in other species. In the human embryo, hypoblast differentiation has not previously been characterised. Here we demonstrate co-exclusive immunolocalisation of Nanog and Gata4 in human blastocysts, implying segregation of epiblast and hypoblast, as in rodent embryos. However, the formation of hypoblast in the human is apparently not dependent upon FGF signalling, in contrast to rodent embryos. Nonetheless, the persistence of Nanog-positive cells in embryos following treatment with FGF inhibitors is suggestive of a transient naïve pluripotent population in the human blastocyst, which may be similar to rodent epiblast and ES cells but is not sustained during conventional human ES cell derivation protocols.
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