The changes of sugar, alcohol and acid were investigated during the wine fermentation. The effect of the inoculum of yeast and the top-space of fermentor on the wine fermentation was also studied. The result showed that mulberry could be used for making a high quality fruit wine. The inoculum of yeast affected the starting time of mulberry wine fermentation, whereas it did not affect the changes of sugar, alcohol and acid significantly; the top-space of fermentor only affected the acid production, and had no significant effect on the changes of other compositions.