Identification of Multiple Alleles at the Wx Locus and Development of Single Segment Substitution Lines in Rice

The microsatellite markers 484/485 and 484/W2R were used to identify the multiple alleles at the Wx locus in rice germplasm. Fifteen alleles were identified in 278 accessions by the use of microsatellite class and G-T polymorphism. Among them, (CT)_ 12-G, (CT)_ 15-G, (CT)_ 16-G, (CT)_ 17-G, (CT)_ 18-G and (CT)_ 21-G are new ones. Seventy-two single segment substitution lines (SSSLs) carrying different alleles at the Wx locus were developed by the use of Huajingxian 74 with the (CT)_ 11-G allele as a recipient and 20 varieties containing 12 alleles at the Wx locus as donors. The estimated length of the substituted segments ranged from 2.2 cM to 77.3 cM with an average of 17.4 cM.