ANTICANCER & CYTOTOXIC POTENTIAL OF AQUEOUS EXTRACT OF TRITICUM AESTIVUM ON HELA CELL LINE

The objective of the study was to analyze the anticancer property of the leaves of Triticum aestivum on HeLa cells. The Indian medicinal plant Triticum aestivum that is used in traditional medicine for cancer and non cancerous diseases was collected. The crude aqueous extract was prepared by using standard protocols. The antiproliferative effect of the aqueous extract was evaluated in vitro by employing MTT assay. The potency of each plant extract concentration was calculated in terms of percent cell inhibition of VERO and HeLa cells. The extract showed dose dependent anticancer activity on the cancer cell line i.e HeLa cell line while the extract did not show any cell toxic potential to the normal cell line i.e. Vero cell line. . The MTT assay showed an anti proliferative activity (IC50) for the HeLa cell line at 133.6 μg/ml of crude extract. Keywords: Triticum aestivum, HeLa cells, VERO Cell line, MTT assay, cytotoxic, aqueous extract