Chemical constituents in root bark of Changium smyrnioides

Objective To study the chemical constituents from the root bark of Changium smyrnioides. Methods Compounds were isolated by various kinds of column chromatographies on silica gel, Sephadex LH-20, and recycling preparative HPLC from the ethanol extract in the root bark of C. smyrnioides, and their structures were elucidated by the physicochemical characteristics and spectral analyses. Results Fifteen chemical constituents were obtained and identified as imperatorin(1), phellopterin(2), xanthotoxol(3), 5-hydroxy-8-methoxy-psoralen(4), vanillic acid(5), alloimperatorin(6), psoralen(7), bergapten(8), 8-O-β-D-glucopyranosyl-5-methoxylpsoralen(9), isopimpinellin(10), caffeic acid(11), aurantiamide acetate(12), vaginatin(13), β-sitosterol(14), and succinic acid(15). Conclusion Compounds 6—13 are isolated from the plants in Changium Wolff for the first time.