Determination of malachite green by flow injection potassium ferricyanide-calcein chemiluminescence

In alkaline media,malachite green could enhance the chemiluminescence(CL) of potassium ferricyanide and calcein greatly.The relative CL intensity was linear with malachite green concentration over certain scope.Based on the observation,a new method for the determination of malachite green has been established by chemilumines cence combined with flow injection technique.Under the optimum conditions,the linear response range of this method was 3.0×10-8-1.0×10-6g/mL and the detection limit was 2.5×10-9g/mL.The relative standard deviation(RSD) was 2.3% for the 11 replicated determinations of 1.0×10-7g/mL malachite green.