草酸钙
细胞内
化学
坏死性下垂
氧化应激
肾
钙
生物学中的钙
污渍
草酸盐
蛋白质组
细胞
生物化学
生物物理学
细胞凋亡
程序性细胞死亡
医学
生物
内科学
有机化学
基因
作者
Arada Vinaiphat,Siripat Aluksanasuwan,Juthatip Manissorn,Suchitra Sutthimethakorn,Visith Thongboonkerd
出处
期刊:Proteomics
[Wiley]
日期:2017-07-18
卷期号:17 (15-16): 1700192-1700192
被引量:30
标识
DOI:10.1002/pmic.201700192
摘要
We have previously identified changes in the cellular proteome of renal tubular cells induced by low-dose (100 μg/mL) and high-dose (1000 μg/mL) calcium oxalate monohydrate (COM) and dihydrate (COD) crystals. However, the functional significance of such expression data remained unclear. In this study, we performed comparative analyses and functional investigations of four proteomic datasets to define potential mechanisms by which renal tubular cells responded to differential crystal types and doses. The data showed that high-dose induced greater changes than low-dose, whereas COM induced more changes than COD. Luciferin–luciferase ATP assay revealed increased intracellular ATP level by high-dose of both COM and COD. OxyBlot assay and Western blotting showed accumulated intracellular oxidized proteins but decreased ubiquitinated proteins by high-dose of both crystals. Flow cytometric analysis of cell death showed that high-dose of both crystals, particularly COM, significantly increased cell death. Also, crystal adhesion assay showed higher degree of cell–crystal adhesion in high-dose and COM when compared to low-dose and COD, respectively. Finally, pretreatment of epigallocatechin-3-gallate revealed a protective effect on COM/COD crystals-induced oxidative stress and cell-crystal adhesion. Collectively, these data may provide a better understanding of cellular responses of renal tubular cells to COM/COD crystals in kidney stone disease.
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