Fluid platelet‐rich fibrin stimulates greater dermal skin fibroblast cell migration, proliferation, and collagen synthesis when compared to platelet‐rich plasma

Abstract Background Regenerative therapies in the field of facial aesthetics have become a growing field of interest with many recent advancements made over the past decade to meet the growing worldwide demand. While first versions of platelet‐derived concentrates were formulated with anticoagulants (PRP), recent modifications to centrifugation speeds and times have permitted the development of a liquid platelet‐rich fibrin (fluid‐PRF) without use of anticoagulants. Objective To compare this entirely natural platelet concentrate (fluid‐PRF) to formally utilized PRP on skin cell behavior and regeneration. Methods Dermal skin fibroblast was cultivated with either fluid‐PRF or PRP and investigated for their ability to promote/influence cell viability, migration, spreading, proliferation, and mRNA levels of known mediators of dermal biology including PDGF, TGF‐beta, and fibronectin. Results All platelet concentrates were nontoxic to cells demonstrating high cell survival. Skin fibroblasts migrated over 350% more in fluid‐PRF when compared to control and PRP (200% increase). Fluid‐PRF also significantly induced greater cell proliferation at 5 days. While both PRP and fluid‐PRF induced significantly elevated cell mRNA levels of PDGF, it was observed that TGF‐beta, collagen 1, and fibronectin mRNA levels were all significantly highest in the fluid‐PRF group. Lastly, fluid‐PRF demonstrated a significantly greater ability to induce collagen matrix synthesis when compared to PRP. Conclusion The findings from the present study demonstrate greater regenerative potential of fluid‐PRF on human skin fibroblasts. Future clinical use of fluid‐PRF in the field of facial aesthetics is necessary to further evaluate the potential advantages of anticoagulant removal from platelet concentrates.