Cloning and analysis of the Glwc‐1 and Glwc‐2 genes encoding putative blue light photoreceptor from Ganoderma lucidum

Blue light plays an important role during the growth of Ganoderma lucidum , one of the best‐known medicinal macrofungi in China. In the present study, we cloned Glwc‐1 and Glwc‐2 , the homologue of the blue light photoreceptors Ncwc‐1 and Ncwc‐2 of Neurospora crassa , from G. lucidum . The deduced amino acid sequence of Glwc‐1 contained the similar function domains as NcWC‐1 including LOV, PAS B, PAS C, and PAC domains. The deduced amino acid sequence of Glwc‐2 contained PAS domain and GATA‐type zinc finger (Znf) domain as well as NcWC‐2. Phylogenetic analysis based on fungal WC‐1 and WC‐2 supported GlWC‐1 and GlWC‐2 were blue light receptors. The expression of Glwc‐1 and Glwc‐2 indicated that they might play an important role during the primordium differentiation process of G. lucidum , and the external blue light stimulation increased the expression of Glwc‐1 and Glwc‐2 .