P003 The Effect of Centella Asiatica on Inactive Frequency and Movement Distance in Insomnia Zebrafish (Danio rerio) Larvae Model

Abstract Introduction Insomnia is a global health problem. Zebrafish (Danio rerio) is an ideal model for studying sleep disorder due to its diurnal cycle. Centella Asiatica (CA) is thought to have an anti-insomnia effect, but its mechanism is not clearly understood. The aim of this study is to evaluate the effect of CA on insomnia zebrafish larvae model by observing sleep parameters including inactive frequency and movement distance. Methods This experimental study is divided into five groups of zebrafish larvae consisting of control group, insomnia group with 24 hours of light exposure for 7 days, insomnia group with CA concentration of 2.5, 5, and 10 µg/mL. Observation of zebrafish larval movement was carried out on 5, 6, and 7 dpf (day post fertilization) during 30 minutes before and after turning off light exposure. Results There were significant differences on 5, 6, and 7 dpf between the normal group, insomnia group, and all treatment groups with CA addition on inactive frequency and movement distance in light and dark conditions (p<0.05). Discussion This study showed that CA has an anti-insomnia effect. CA can improve sleep quality of insomnia zebrafish larvae model by increasing inactive frequency and shortening movement distance. Keywords Centella Asiatica, zebrafish, inactive frequency, movement distance.