自愈水凝胶
软骨
细胞包封
再生(生物学)
透明质酸
软骨细胞
活力测定
组织工程
生物医学工程
材料科学
化学
细胞外基质
生物物理学
细胞
解剖
细胞生物学
高分子化学
医学
生物化学
生物
作者
Jijo Thomas,Vianni Chopra,Swati Rajput,Rajdeep Guha,Naibedya Chattopadhyay,Deepa Ghosh
出处
期刊:Biomacromolecules
[American Chemical Society]
日期:2023-06-28
卷期号:24 (7): 3313-3326
被引量:2
标识
DOI:10.1021/acs.biomac.3c00351
摘要
Injectable hydrogels have demonstrated advantages in cartilage repair by enabling the delivery of cells through a minimally invasive approach. However, several injectable hydrogels suffer from rapid degradation and low mechanical strength. Moreover, higher mechanical stiffness in hydrogels can have a detrimental effect on post-implantation cell viability. To address these challenges, we developed an in situ forming bioinspired double network hydrogel (BDNH) that exhibits temperature-dependent stiffening after implantation. The BDNH mimics the microarchitecture of aggrecan, with hyaluronic acid-conjugated poly(N-isopropylacrylamide) providing rigidity and Schiff base crosslinked polymers serving as the ductile counterpart. BDNHs exhibited self-healing property and enhanced stiffness at physiological temperature. Excellent cell viability, long time cell proliferation, and cartilage specific matrix production were observed in the chondrocytes cultured in the BDNH hydrogel. Evidence of cartilage regeneration in a rabbit cartilage defect model using chondrocyte-laden BDNH has suggested it to be a potential candidate for cartilage tissue engineering.
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