化学
一氧化氮
溶酶体
荧光
选择性
部分
生物物理学
吗啉
组合化学
光化学
生物化学
立体化学
有机化学
催化作用
量子力学
生物
酶
物理
作者
Zhiling Xu,Songtao Liu,Liren Xu,Zichun Li,Xiaoli Zhang,Hao Kang,Lei Zhu,Jin Yu,Jing Jing,Guangle Niu,Xiaoling Zhang
标识
DOI:10.1016/j.aca.2024.342303
摘要
Nitric oxide (NO) plays critical roles in both physiology and pathology, serving as a significant signaling molecule. Recent investigations have uncovered the pivotal role of lysosome as a critical organelle where intracellular NO exists and takes function. In this study, we developed a novel ratiometric fluorescent probe called XL-NO and modified it with a morpholine unit, which followed the intramolecular charge transfer (ICT) mechanism. The probe could detect lysosomal nitric oxide with high selectivity and sensitivity. The probe XL-NO contained a secondary amine moiety that could readily react with NO in lysosomes, leading to the formation of the N-nitrosation product. The N-nitroso structure enhanced the capability in push-pull electron, which obviously led to the change of fluorescence from 621 nm to 521 nm. In addition, XL-NO was discovered to have some evident advantages, such as significant ratiometric signal (I521/I621) change, strong anti-interference ability, good biocompatibility, and a low detection limit (LOD = 44.3 nM), which were crucial for the detection of lysosomal NO. To evaluate the practical application of XL-NO, NO imaging experiments were performed in both living cells and zebrafish. The results from these experiments confirmed the feasibility and reliability of XL-NO for exogenous/endogenous NO imaging and lysosome targeting.
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