精子发生
生物
无精子症
生殖细胞
体细胞
基因
细胞
支持细胞
男性不育
遗传学
不育
内分泌学
怀孕
作者
Xiaolong Wu,Mengji Lu,Damin Yun,Sheng Gao,Fei Sun
标识
DOI:10.1093/qjmed/hcae009
摘要
Abstract Background: High-throughput single-cell RNA sequencing (scRNA-seq) is widely used in spermatogenesis. However, it only reveals short reads in germ and somatic cells, limiting the discovery of novel transcripts and genes. Aim This study shows the long-read transcriptional landscape of spermatogenesis in obstructive azoospermia (OA) and Sertoli cell-only (SCO) patients. Design Single cells were isolated from testicular biopsies of OA and NOA patients. Cell culture was identified by comparing Pacbio long-read single-cell sequencing (OA n = 3, NOA n = 3) with short-read scRNA-seq (OA n = 6, NOA n = 6). Ten germ cell types and eight somatic cell types were classified based on known markers. Methods Pacbio long read single-cell sequencing, short-read scRNA-seq, Polymerase Chain Reaction. Results A total of 130,426 long-read transcripts (100,517 novel transcripts and 29,909 known transcripts) and 49,508 long-read transcripts (26,002 novel transcripts, and 23,506 known transcripts) have been detected in OA and NOA patients, respectively. Moreover, 36,373 and 1642 new genes are identified in OA and NOA patients, respectively. Importantly, specific expressions of long-read transcripts were detected in germ and stomatic cells during normal spermatogenesis. Conclusion We have identified total full-length transcripts in OA and NOA, and new genes were found. Furthermore, specific expressed full-length transcripts were detected, and the genomic structure of transcripts was mapped in different cell types. These findings may provide valuable information on human spermatogenesis and the treatment of male infertility.
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