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Processing parameters, techno-functional properties and potential food application of lentil protein concentrate as an ingredient for the plant-based market

成分 食品科学 大米蛋白 萃取(化学) 化学 植物蛋白 豌豆蛋白 豆类 溶解度 食物蛋白 蛋白质纯化 色谱法 农学 生物 有机化学
作者
Rodrigo Coppe Caldeira,Lucas de Paiva Gouvêa,Janice Ribeiro Lima,Carmine Conte,Daniela de Grandi Castro Freitas de Sá,Melícia Cintia Galdeano,Ilana Felberg,Janice Ribeiro Lima,Caroline Mellinger‐Silva
出处
期刊:Food Research International [Elsevier BV]
卷期号:189: 114569-114569
标识
DOI:10.1016/j.foodres.2024.114569
摘要

Lentil (Lens culinaris) is a protein-rich legume consumed worldwide and it also has the potential to become an alternative source of protein ingredient for human nutrition. The aim of this study was to determine the best processing parameters for the whole grain protein wet extraction, as well as to analyze the techno-functional properties, and physical characteristics of the protein concentrate and its flour. It was also evaluated the application of the concentrate into a fish-like croquette. The processing route was carried out by alkaline extraction and acid precipitation of the proteins where the pH, stirring time and solute:solvent ratio were evaluated. The final dried protein concentrate presented 85% protein on dry basis and a mass yield of 14%. The results were reproducible when tested on a first scaling up test. For the techno-functional properties, solubility, water and oil retention capacities, emulsification and foaming capacities and stability, and gelling capacity were tested. As for the food application into fish-like croquettes, the lentil protein showed similar scores for sensory acceptance, flavor and texture when compared to a commercial clean-taste concentrate. The results observed in this study were compatible to other alternative pulse-protein ingredients on the market, positioning lentil protein as a promising alternative protein source to produce ingredients for the plant-based market.

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