单克隆抗体
抗原
有限元法
生物传感器
抗体
生物
免疫学
材料科学
纳米技术
工程类
结构工程
作者
Pelin Akcalı,Kübra Kelleci,Sevil Özer
出处
期刊:Current Protein & Peptide Science
[Bentham Science]
日期:2024-03-01
卷期号:25 (3): 256-266
标识
DOI:10.2174/0113892037259122231013153546
摘要
Biosensors and MEMS have witnessed rapid development and enormous interest over the past decades. Constant advancement in diagnostic, medical, and chemical applications has been demonstrated in several platforms and tools. In this study, the analytical and FEA of the microcantilever used in biomolecular analyses were compared with the experimental analysis results.In this study, MITF antigen, which is a melanoma biomarker, and anti-MITF antibody (D5) were selected as biomolecules. A MEMS-type microcantilever biosensor was designed by functionalizing the AFM cantilever by utilizing the specific interaction dynamics and intermolecular binding ability between both molecules. Surface functionalization of cantilever micro biosensors was performed by using FEA. The stress that will occur as a result of the interactions between the MITF-D5 has been determined from the deviation in the resonant frequency of the cantilever.It has been found that the simulation results are supported by analytical calculations and experimental results.The fact that the results of the simulation study overlap with the experimental and mathematical results allows us to get much cheaper and faster answers compared to expensive and time-consuming experimental approaches.
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